Download Ancient DNA: Methods and Protocols by Tara L. Fulton (auth.), Beth Shapiro, Michael Hofreiter PDF

By Tara L. Fulton (auth.), Beth Shapiro, Michael Hofreiter (eds.)

Research into historical DNA started greater than 25 years in the past with the e-book of brief mitochondrial DNA series fragments from the quagga, an extinct relative of the zebra. historical DNA study particularly won momentum following the discovery of PCR, which allowed hundreds of thousands of copies to be made up of the few ultimate DNA molecules preserved in fossils and museum specimens. In Ancient DNA: equipment and Protocols specialist researchers within the box describe some of the protocols which are now known to check historic DNA. those contain directions for constructing an old DNA laboratory, extraction protocols for quite a lot of diverse substrates, info of laboratory strategies together with PCR and NGS library education, and proposals for applicable analytical ways to make experience of the sequences got. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and key pointers on troubleshooting and warding off identified pitfalls.

Authoritative and useful, Ancient DNA: equipment and Protocols seeks to assist scientists within the additional research of historic DNA and the methodological techniques in old research.

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Discard the lower, chloroform layer (see Note 8). 8. 1 volume 3 M sodium acetate (approx. pH 5). , Austin, TX), following the manufacturers’ guidelines. Mix well (see Note 9). 9. Immediately centrifuge at high speed (>10,000 × g) for 30 min at room temperature. 10. Immediately following centrifugation, decant the liquid from the tube carefully. The DNA will have precipitated into a pellet at the bottom of the tube and may not be visible. 11. To rinse the pellet, gently add 500–1,000 mL 85% ethanol, slowly invert the tube once, then centrifuge for 5 min at high speed.

However, deposits of megafaunal dung, namely that of the extinct ground sloth Nothrotheriops shastensis found in caves of the American southwest, rival in extent the vast deposits of mammoth bone and teeth in the permafrost (1). Paleofeces are most often found within caves and rock shelters (2), although some have been found at open-air sites. Paleofeces likely make up a large percentage of the sediment found within cave floors, and to some degree, that found within permafrost soils as well. This may explain the success in retrieving the DNA of past inhabitants of caves (3) and megafauna of the high arctic (4).

Forensic Sci Int Genet 1:191–195 Chapter 4 Case Study: Recovery of Ancient Nuclear DNA from Toe Pads of the Extinct Passenger Pigeon * Tara L. Fulton, Stephen M. Wagner, and Beth Shapiro Abstract A variety of DNA extraction methods have been employed successfully to extract DNA from museum specimens. Toe pads are a common source of ancient DNA in birds, as they are generally not an informative character and can be removed without significant destruction of precious specimens. However, the DNA in these tissues is often highly degraded, both by natural postmortem decay and due to treatment by preservatives.

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